9QZL

Structure of NONO bound to (R)-SKBG-1 in P43212

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-12-13
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9382
Spacegroup name	P 43 21 2
Unit cell lengths	87.069, 87.069, 138.587
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.870 - 2.900
R-factor	0.2068
Rwork	0.203
R-free	0.27120
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.049
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.190
High resolution limit [Å]	2.900	2.900
Rmerge	0.351	0.700
Rpim	0.116	0.230
Number of reflections	22579	5661
<I/σ(I)>	6.05
Completeness [%]	100.0	100
Redundancy	10
CC(1/2)	0.972	0.683

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.2 M Ammonium acetate, 0.1 M HEPES pH 7.5 or Tris-HCl pH 8.5, 25 % w/v PEG 3350