9QYY
MINPP1 from Bacteroides thetaiotaomicron R183 mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9690 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.383, 119.123, 76.031 |
| Unit cell angles | 90.00, 107.96, 90.00 |
Refinement procedure
| Resolution | 30.760 - 2.360 |
| R-factor | 0.1665 |
| Rwork | 0.164 |
| R-free | 0.22000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.864 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.760 | 2.444 |
| High resolution limit [Å] | 2.360 | 2.360 |
| Number of reflections | 36084 | 3566 |
| <I/σ(I)> | 7.58 | 3.15 |
| Completeness [%] | 98.7 | |
| Redundancy | 3 | 3 |
| CC(1/2) | 0.989 | 0.773 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 289 | 0.2 M ammonium acetate pH 5.0 18 % (w/v) PEG 3350 |
