9QXZ
Structure of human NONO bound to (R)-SKBG-1 in p212121
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-10-29 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9785 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.258, 127.098, 201.445 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.990 - 2.500 |
| R-factor | 0.2005 |
| Rwork | 0.198 |
| R-free | 0.25330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.978 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.21_5207: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.000 | 2.490 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.010 | 1.166 |
| Rmeas | 0.012 | |
| Rpim | 0.049 | 1.340 |
| Number of reflections | 611051 | 20473 |
| <I/σ(I)> | 9.89 | 1 |
| Completeness [%] | 99.5 | 97.3 |
| Redundancy | 5.4 | |
| CC(1/2) | 0.998 | 0.431 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2 M Ammonium acetate, 0.1 M HEPES pH 7.5 or Tris-HCl pH 8.5, 25 % w/v PEG 3350 |
