9QWR

MIC60 helical bundle dimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-05-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	P 41 21 2
Unit cell lengths	76.120, 76.120, 168.410
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.160 - 2.780
R-factor	0.2474
Rwork	0.245
R-free	0.28310
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.482
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.160	2.880
High resolution limit [Å]	2.780	2.780
Rmerge	0.137	3.240
Number of reflections	13089	2028
<I/σ(I)>	12.48
Completeness [%]	99.6
Redundancy	11.3
CC(1/2)	0.990	0.300

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.2 M Ammonium citrate, 20% PEG 3350,