9QW2
Crystal structure of human lung surfactant protein D trimeric fragment with bound synthetic HepII-HepI-PhosI ligand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-09 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.439, 108.179, 55.650 |
| Unit cell angles | 90.00, 90.90, 90.00 |
Refinement procedure
| Resolution | 55.493 - 1.920 |
| Rwork | 0.180 |
| R-free | 0.21190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.566 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.640 | 1.970 |
| High resolution limit [Å] | 1.920 | 1.920 |
| Rmerge | 0.078 | 0.305 |
| Number of reflections | 49393 | 3267 |
| <I/σ(I)> | 8 | 3 |
| Completeness [%] | 98.8 | 98 |
| Redundancy | 2.6 | |
| CC(1/2) | 0.973 | 0.788 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 16% PEG 4000 and 0.1 M Tris pH 8.0 |
