9QVI
Central domain of Glucan Water Dikinase-1 in alternative closed conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-05-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.8856 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 94.134, 94.134, 150.757 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.111 - 3.250 |
| Rwork | 0.261 |
| R-free | 0.30610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.959 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.890 | 3.430 |
| High resolution limit [Å] | 3.250 | 3.250 |
| Rmerge | 0.046 | 0.749 |
| Number of reflections | 11258 | 1578 |
| <I/σ(I)> | 5 | 1 |
| Completeness [%] | 100.0 | |
| Redundancy | 12.7 | |
| CC(1/2) | 0.999 | 0.966 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 285 | 0.1 M HEPES Na salt pH 7.5, 0.7 M tri-Na citrate |
