9QT3

Structure of vaccinia virus A26 (residues 1-397) in complex with Fab 8M2110

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-10-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.978565
Spacegroup name	P 32 2 1
Unit cell lengths	119.405, 119.405, 632.812
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.080 - 2.900
R-factor	0.2023
Rwork	0.201
R-free	0.23570
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.503
Data reduction software	XDS
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.080	2.950
High resolution limit [Å]	2.900	2.900
Number of reflections	223523	5717
<I/σ(I)>	8.6
Completeness [%]	100.0
Redundancy	18
CC(1/2)	0.987	0.638

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	20% (w/v) PEG 3350, 0.2 M ammonium citrate