9QT1

Bacterial histone HLp from Leptospira perolatii bound to DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-08-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	P 41 21 2
Unit cell lengths	67.934, 67.934, 97.902
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.160 - 2.100
R-factor	0.2513
Rwork	0.250
R-free	0.27946
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.297
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0266)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.160	2.230
High resolution limit [Å]	2.100	2.100
Rmeas	0.081	0.743
Number of reflections	13927	2187
<I/σ(I)>	11.09	1.96
Completeness [%]	99.7	99.5
Redundancy	5.62	5.53
CC(1/2)	0.999	0.840

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1 M HEPES, PH 7.5 and 25% PEG 6000