9QT0
DNA-binding bacterial histone HLp from Leptospira perolatii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-03-24 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 55.700, 55.700, 58.463 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.250 - 1.300 |
| R-factor | 0.17717 |
| Rwork | 0.175 |
| R-free | 0.21080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.164 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0266) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.250 | 1.380 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmeas | 0.052 | 1.400 |
| Number of reflections | 26187 | 4107 |
| <I/σ(I)> | 19.87 | 1.14 |
| Completeness [%] | 99.7 | 98.3 |
| Redundancy | 9.43 | 5.34 |
| CC(1/2) | 0.999 | 0.380 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 0.1 M Citric acid, pH 5.0 and 3.15 M Ammonium sulfate |
