9QS9
Structure and mechanism of the broad spectrum CRISPR-associated ring nuclease Crn4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-07-05 |
| Detector | DECTRIS EIGER2 S 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.271, 102.899, 72.442 |
| Unit cell angles | 90.00, 103.88, 90.00 |
Refinement procedure
| Resolution | 70.425 - 1.441 |
| Rwork | 0.222 |
| R-free | 0.23950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.987 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 102.900 | 1.470 |
| High resolution limit [Å] | 1.440 | 1.440 |
| Rmerge | 0.068 | 2.101 |
| Number of reflections | 169690 | 8333 |
| <I/σ(I)> | 11.2 | |
| Completeness [%] | 100.0 | |
| Redundancy | 6.9 | |
| CC(1/2) | 0.998 | 0.299 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 23.8% PEG3350, 0.2M magnesium chloride, 0.1M bis-Tris pH 5.5 |
