9QRF
Ancestral protein of diDNase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-09-05 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 1.033210 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 70.903, 70.948, 48.241 |
| Unit cell angles | 90.00, 115.81, 90.00 |
Refinement procedure
| Resolution | 47.450 - 1.450 |
| R-factor | 0.1674 |
| Rwork | 0.166 |
| R-free | 0.20040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.908 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.450 | 1.502 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.068 | 0.765 |
| Number of reflections | 36862 | 25793 |
| <I/σ(I)> | 14.42 | |
| Completeness [%] | 96.7 | 95.28 |
| Redundancy | 6.9 | 7.1 |
| CC(1/2) | 1.000 | 0.976 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 292 | 0.1M MES (pH 5), 20% (w/v) PEG 6000 |
