9QR7

Structural characterization of a N-acetylmuramic acid-binding site in human RNase2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-07-09
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	41.674, 52.860, 56.439
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.310 - 1.010
R-factor	0.1329
Rwork	0.132
R-free	0.14890
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.883
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHENIX
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.310	1.040
High resolution limit [Å]	1.010	1.010
Number of reflections	55704	467
<I/σ(I)>	1.34
Completeness [%]	85.5
Redundancy	5.4
CC(1/2)	0.999	0.894

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.22M Sodium formate 0.1M Bis-Tris propane 12% PEG 3350 PH=7