9QQV
Structure of the F13 protein of Vaccinia virus in complex with G243-1720
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-01-31 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.978564977646 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 281.495, 281.495, 281.495 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.420 - 3.400 |
| R-factor | 0.2283 |
| Rwork | 0.226 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.435 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.420 | 3.670 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Number of reflections | 13691 | 2755 |
| <I/σ(I)> | 17.7 | |
| Completeness [%] | 99.9 | |
| Redundancy | 78.7 | |
| CC(1/2) | 1.000 | 0.763 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 1 M Na3 citrate, 0.1 M Imidazole pH 8 (cryoprotected with 33% glycerol, soaked with 1 mM G243-1720) |
