9QQO
Crystal structure of a beta-glycosidase from Prevotella sp.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM07 |
| Synchrotron site | ESRF |
| Beamline | BM07 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-04-26 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979510 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 134.182, 139.490, 172.660 |
| Unit cell angles | 90.00, 99.84, 90.00 |
Refinement procedure
| Resolution | 114.280 - 2.500 |
| R-factor | 0.1698 |
| Rwork | 0.169 |
| R-free | 0.18230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.907 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 139.490 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.151 | 0.576 |
| Rmeas | 0.172 | 0.664 |
| Rpim | 0.081 | 0.325 |
| Number of reflections | 209766 | 10526 |
| <I/σ(I)> | 10.2 | 2.5 |
| Completeness [%] | 97.1 | 98.4 |
| Redundancy | 4.3 | |
| CC(1/2) | 0.945 | 0.752 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% w/v polyethylene glycol 8000, 100 mM CAPS/sodium hydroxide, pH 10.5, and 200 mM sodium chloride |
