9QQ9

Crystal structure of the relaxase domain of RelpLS20, prototype of MobL family of relaxases

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979190
Spacegroup name	P 61
Unit cell lengths	100.918, 100.918, 58.875
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.030 - 1.660
R-factor	0.1731
Rwork	0.172
R-free	0.19850
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.023
Data reduction software	XDS (20220110)
Data scaling software	XSCALE (20220110)
Phasing software	PHENIX (1.16)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.900	1.750
High resolution limit [Å]	1.660	1.660
Rmeas	0.069	2.760
Rpim	0.022	0.872
Number of reflections	38321	5907
<I/σ(I)>	15	1.1
Completeness [%]	94.2	99.9
Redundancy	9.8	9.9
CC(1/2)	0.998	0.671

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	291	1ul of protein at 6mg/ml added to 1ul reservoir (100mM Tris pH9, 22%PEG 6000)