9QQ8
Crystal structure of the catalytic N-terminal domain of the relaxase of plasmid pLS20 in complex with the core region
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979181 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 78.680, 78.680, 158.560 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.760 - 2.290 |
| R-factor | 0.2131 |
| Rwork | 0.212 |
| R-free | 0.23940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.142 |
| Data reduction software | XDS (20220110) |
| Data scaling software | XSCALE (20220110) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.763 | 2.430 |
| High resolution limit [Å] | 2.290 | 2.290 |
| Rmeas | 0.104 | |
| Number of reflections | 24561 | 3869 |
| <I/σ(I)> | 11.58 | 0.78 |
| Completeness [%] | 99.5 | 97.2 |
| Redundancy | 7.38 | |
| CC(1/2) | 0.998 | 0.550 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 1.6 M Ammonium sulfate, 0.1 M Sodium HEPES pH 7.5, 0.1 M Sodium chloride |
