9QOY

Pseudomonas aeruginosa APH(3")-IIb - Wild Type

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-04
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.8731281579802835
Spacegroup name	C 2 2 21
Unit cell lengths	83.006, 110.127, 123.673
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.350 - 1.792
Rwork	0.180
R-free	0.22780
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XDS
Refinement software	PHENIX ((1.21.1_5286: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.350	1.820
High resolution limit [Å]	1.790	1.790
Number of reflections	53073	4934
<I/σ(I)>	10.44
Completeness [%]	99.0	96.2
Redundancy	2
CC(1/2)	0.995	0.700

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	291.15	0.1 M HEPES pH 7.4, 0.6 M tri-Sodium Citrate, 0.025 M KCl micro seeding