9QN7

Structure of talin in complex with a peptide fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-09-14
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	0.9537
Spacegroup name	P 43 21 2
Unit cell lengths	84.940, 84.940, 346.330
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	76.260 - 2.760
R-factor	0.2589
Rwork	0.257
R-free	0.29580
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.950
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	86.580	2.890
High resolution limit [Å]	2.760	2.760
Rmerge	0.441	3.087
Rpim	0.087	0.590
Number of reflections	33905	4373
<I/σ(I)>	10.6
Completeness [%]	99.9	100
Redundancy	26.2	27.7
CC(1/2)	0.999	0.483

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.4	294.15	0.9M sodium potassium tartrate tetrathydrate, 20% w/v glycerol, 0.05M HEPES pH 7.4