9QN4
Fe(II)-2-oxoglutarate-dependent pseudomonal iron uptake factor C in complex with 2-oxoglutarate (Fe)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-07-22 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97628 |
| Spacegroup name | P 1 |
| Unit cell lengths | 80.605, 80.113, 88.798 |
| Unit cell angles | 63.12, 62.94, 59.76 |
Refinement procedure
| Resolution | 44.000 - 1.850 |
| R-factor | 0.2277 |
| Rwork | 0.227 |
| R-free | 0.24350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.753 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.000 | 1.880 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.058 | 0.585 |
| Rmeas | 0.079 | 0.827 |
| Rpim | 0.041 | 0.585 |
| Number of reflections | 132675 | 6592 |
| <I/σ(I)> | 8.9 | 1.7 |
| Completeness [%] | 95.0 | 95.7 |
| Redundancy | 3.8 | 3.8 |
| CC(1/2) | 0.996 | 0.619 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 290 | 0.1M succinic acid, sodium phosphate monobasic monohydrate, glycine (pH 6) 30% PEG 1000 2 mM FeCl2 |
