9QN2
Fe(II)-2-oxoglutarate-dependent pseudomonal iron uptake factor C in complex with Malate (Mn)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-01-26 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 1 |
| Unit cell lengths | 80.585, 80.577, 88.945 |
| Unit cell angles | 63.06, 63.07, 60.07 |
Refinement procedure
| Resolution | 44.090 - 1.900 |
| R-factor | 0.1797 |
| Rwork | 0.179 |
| R-free | 0.20330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.935 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.090 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.079 | 0.984 |
| Rmeas | 0.090 | 1.144 |
| Rpim | 0.063 | 0.582 |
| Number of reflections | 126101 | 6164 |
| <I/σ(I)> | 18.5 | 1.4 |
| Completeness [%] | 92.5 | 87.4 |
| Redundancy | 3.7 | 3.7 |
| CC(1/2) | 0.998 | 0.487 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 290 | 0.1M DL-Malic acid / MES monohydrate (pH 8.0) 30% PEG 1000 |
