9QN1
Fe(II)-2-oxoglutarate-dependent pseudomonal iron uptake factor C in complex with N-Oxalylglycine (Mn)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-04-25 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97627 |
| Spacegroup name | P 1 |
| Unit cell lengths | 80.833, 80.761, 89.099 |
| Unit cell angles | 116.97, 90.05, 119.94 |
Refinement procedure
| Resolution | 44.170 - 1.850 |
| R-factor | 0.1788 |
| Rwork | 0.177 |
| R-free | 0.20920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.827 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.170 | 1.880 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.045 | 0.718 |
| Rmeas | 0.063 | 0.852 |
| Rpim | 0.045 | 0.602 |
| Number of reflections | 137046 | 6904 |
| <I/σ(I)> | 11.5 | 1.5 |
| Completeness [%] | 96.5 | |
| Redundancy | 3.5 | 3.6 |
| CC(1/2) | 0.998 | 0.671 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 290 | 0.1 M Na HEPES, pH7.5 27 % v/v PEG 600 |
