9QMZ
Arabidopsis thaliana 4-hydroxyphenylpyruvate dioxygenase in complex with Fenquinotrione (Mn)
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-01-14 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97628 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 77.103, 83.445, 61.250 |
| Unit cell angles | 90.00, 102.79, 90.00 |
Refinement procedure
| Resolution | 35.560 - 1.550 |
| R-factor | 0.1621 |
| Rwork | 0.161 |
| R-free | 0.18140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.415 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.21.2_5419: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.560 | 1.580 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.079 | 1.122 |
| Rmeas | 0.086 | 1.216 |
| Rpim | 0.032 | 0.463 |
| Number of reflections | 54361 | 2718 |
| <I/σ(I)> | 11.1 | |
| Completeness [%] | 99.3 | 99.4 |
| Redundancy | 7 | 6.8 |
| CC(1/2) | 0.999 | 0.739 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 290 | Morpheus F4 0.12 M Monosaccharides 0.1 M Buffer System 1 6.5 37.5 % v/v Precipitant Mix 4 |
