9QMP

E.coli seryl-tRNA synthetase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1989-07-01
Detector	MAR scanner 180 mm plate
Wavelength(s)	0.96
Spacegroup name	C 1 2 1
Unit cell lengths	144.648, 90.720, 69.800
Unit cell angles	90.00, 117.75, 90.00

Refinement procedure

Resolution	19.020 - 2.480
R-factor	0.1516
Rwork	0.150
R-free	0.18580
Structure solution method	MIRAS
RMSD bond length	0.003
RMSD bond angle	0.644
Data reduction software	MOSFLM
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.020	2.580
High resolution limit [Å]	2.480	2.480
Rmerge	0.059	0.250
Number of reflections	26616	1980
<I/σ(I)>	17.1
Completeness [%]	93.7	62.7
Redundancy	2.59

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1% protein solution in 67 mM Tris, 50 mM HCI, 10 mM MgCI2 , 1 mM DTE pH 7.6,1 M ammonium sulphate and 1% octyl-glucoside (practical grade, Pfanstiehl) against 52% saturated ammonium sulphate at room temperature