9QMO

PLK1 PBD apo protein at 1.6A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9998578718850196
Spacegroup name	P 21 21 2
Unit cell lengths	76.700, 98.570, 33.230
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.280 - 1.600
R-factor	0.1989
Rwork	0.197
R-free	0.22840
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.993
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.280	49.280	1.640
High resolution limit [Å]	1.600	7.160	1.600
Rmerge	0.142	0.049	2.828
Rmeas	0.148	0.052	2.937
Number of reflections	34122	456	2461
<I/σ(I)>	10.81
Completeness [%]	100.0
Redundancy	13
CC(1/2)	0.999	0.999	0.261

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.2M Na2H(PO4), 20%PEG3350