9QMD

Crystal Structure of Dimeric Apo-L28H-FNR of A. Fischeri

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97626
Spacegroup name	I 4 2 2
Unit cell lengths	76.090, 76.090, 213.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.050 - 2.100
R-factor	0.1999
Rwork	0.199
R-free	0.22100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.902
Data reduction software	XDS (13/07/2021)
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.050	2.170
High resolution limit [Å]	2.100	2.100
Rmerge	0.056
Number of reflections	18711	1783
<I/σ(I)>	21.4
Completeness [%]	99.3
Redundancy	16.3
CC(1/2)	0.998	0.722

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	MPD, MES, DITHIONITE, ANAEROBIC, PH REMARK 280 6.4