9QM9

TMEM55B tandem RING-like domains in complex with RILPL1 C-terminal peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-07-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9794
Spacegroup name	P 21 21 21
Unit cell lengths	41.514, 48.442, 79.992
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.440 - 1.550
R-factor	0.2067
Rwork	0.205
R-free	0.24620
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.014
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	79.990	1.580
High resolution limit [Å]	1.550	1.550
Rmerge	0.150	2.242
Rmeas	0.163	2.426
Rpim	0.063	0.917
Total number of observations		7789
Number of reflections	24054	1163
<I/σ(I)>	8.9	0.9
Completeness [%]	99.8
Redundancy	6.6	6.7
CC(1/2)	0.998	0.536

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	289.15	0.05M KSCN 0.1M Tris pH 8.5 18% PEG5000/MME