9QM4

Crystal Structure of the flavoprotein monooxygenase RslO9 from Streptomyces bottropensis (alternative N-terminus)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-10-05
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9763
Spacegroup name	C 2 2 21
Unit cell lengths	112.578, 115.998, 104.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.590 - 2.700
R-factor	0.2228
Rwork	0.221
R-free	0.25810
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.529
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.590	2.840
High resolution limit [Å]	2.700	2.700
Number of reflections	19173	2681
<I/σ(I)>	8.19
Completeness [%]	99.7
Redundancy	13.2
CC(1/2)	0.997	0.921

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293.15	41.3%(w/v) PAA2100, 0.1 M HEPES pH 6.5