9QM3
Crystal Structure of the flavoprotein monooxygenase RslO9 from Streptomyces bottropensis (alternative loop conformation)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-02-15 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.976246 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 112.150, 116.770, 104.680 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.430 - 3.100 |
| R-factor | 0.2247 |
| Rwork | 0.222 |
| R-free | 0.27140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.471 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.430 | 3.340 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Number of reflections | 12760 | 2517 |
| <I/σ(I)> | 8.28 | |
| Completeness [%] | 99.5 | |
| Redundancy | 12.9 | |
| CC(1/2) | 0.991 | 0.879 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 M HEPES pH 6.5, 45 %w/v Poly(acrylic acid sodium salt) 2100 |
