9QM2

Crystal Structure of the flavoprotein monooxygenase RslO9 from Streptomyces bottropensis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-09-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.99999
Spacegroup name	C 2 2 21
Unit cell lengths	111.670, 118.230, 104.460
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.240 - 2.400
R-factor	0.2133
Rwork	0.211
R-free	0.25780
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.499
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.240	2.490
High resolution limit [Å]	2.400	2.400
Number of reflections	27359	2684
<I/σ(I)>	8.96
Completeness [%]	99.8
Redundancy	12.2
CC(1/2)	0.996	0.625

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	100 mM HEPES pH 7.0, 300 mM ammonium formate, 20% (w/v) Soklan CP5