9QLJ
Crystal Structure of a Disordered Apo-form of A. Fischeri FNR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979745 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 74.580, 74.580, 207.160 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.890 - 2.500 |
| R-factor | 0.2238 |
| Rwork | 0.223 |
| R-free | 0.23510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.966 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.074 | 3.345 |
| Number of reflections | 10175 | 967 |
| <I/σ(I)> | 19.2 | |
| Completeness [%] | 97.3 | |
| Redundancy | 9.9 | |
| CC(1/2) | 0.999 | 0.207 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | MPD, HEPES, ANAEROBIC, PH 7.1 |
