9QL3

Structure of Rhyzopertha dominica dihydrolipoyl dehydrogenase in the EH2-state at 1.41 angstrom resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-29
Detector	DECTRIS EIGER2 X CdTe 16M
Wavelength(s)	0.97625
Spacegroup name	P 21 21 21
Unit cell lengths	66.280, 91.450, 143.290
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.400 - 1.410
R-factor	0.1669
Rwork	0.166
R-free	0.18250
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.852
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.400	1.450
High resolution limit [Å]	1.410	1.410
Rmeas	0.043	0.840
Number of reflections	166314	12120
<I/σ(I)>	18.58	2.07
Completeness [%]	99.1
Redundancy	4.5
CC(1/2)	0.999	0.680

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	Tris, Magnesium chloride, PEG3350, Formamide