9QKP

Structure of the cyanobacteria specific PRK from Chroococcidiopsis (Hyella disjuncta) PCC 6712 (P41212 crystal form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-07-01
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	0.95374
Spacegroup name	P 41 21 2
Unit cell lengths	92.752, 92.752, 407.667
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	65.590 - 2.430
R-factor	0.2335
Rwork	0.233
R-free	0.26820
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.911
Data reduction software	DIALS
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	84.420	2.470
High resolution limit [Å]	2.430	2.430
Rmerge	0.068	2.004
Rmeas	0.074	2.162
Rpim		0.802
Number of reflections	68603	3310
<I/σ(I)>	16.6
Completeness [%]	100.0	99
Redundancy	7	7.1
CC(1/2)	0.971	0.396

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	289	0.1M Tris-HCl pH 8.0, 28% (w/v) PEG 5000 MME, 0.28 M lithium sulfate