9QKO
Structure of the cyanobacteria specific PRK from Chroococcidiopsis (Hyella disjuncta) PCC 6712 (P1 crystal form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 199 |
| Detector technology | PIXEL |
| Collection date | 2023-07-20 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.619904 |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.572, 53.080, 64.367 |
| Unit cell angles | 92.95, 101.84, 103.24 |
Refinement procedure
| Resolution | 38.490 - 2.600 |
| R-factor | 0.2371 |
| Rwork | 0.235 |
| R-free | 0.27500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.810 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.660 | 2.642 |
| High resolution limit [Å] | 2.597 | 2.597 |
| Rmerge | 0.078 | 0.919 |
| Rmeas | 0.110 | 1.300 |
| Number of reflections | 17524 | 698 |
| <I/σ(I)> | 2.8 | 1 |
| Completeness [%] | 97.3 | 78.6 |
| Redundancy | 1.8 | 1.6 |
| CC(1/2) | 0.983 | 0.370 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 289 | 0.1 M MES-NaOH pH 6.5, 30 % PEG MME 5000, 0.2 M Ammonium sulfate monohydrate |
