9QKN

Structure of the cyanobacteria specific PRK from Gloeocapsa PCC 73106

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-05-26
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.999880
Spacegroup name	I 2 2 2
Unit cell lengths	57.430, 103.470, 140.180
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.590 - 3.200
R-factor	0.259
Rwork	0.256
R-free	0.31610
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.709
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.140	3.260
High resolution limit [Å]	3.200	3.200
Rmerge	0.106	1.275
Rmeas	0.115	1.442
Rpim	0.047	0.660
Number of reflections	7197	343
<I/σ(I)>	9.7	1.4
Completeness [%]	99.8	99.7
Redundancy	6.1
CC(1/2)	0.998	0.673

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	289	0.1 M MES-NaOH pH 6.5 +30 % PEG MME 5000 + 0.2 M Ammonium sulfate monohydrate