9QJ9

Crystal Structure of the Human Retinoid X Receptor DNA-Binding Domain Bound to Trim16 IR1 Response Element

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-09-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 41 21 2
Unit cell lengths	56.019, 56.019, 169.871
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.842 - 3.500
R-factor	0.2702
Rwork	0.267
R-free	0.33520
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	2.315
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (dev_1426)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.842	3.625
High resolution limit [Å]	3.500	3.500
Number of reflections	3712	317
<I/σ(I)>	11.7
Completeness [%]	96.5
Redundancy	2
CC(1/2)	1.000	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		290	20% PEG 1000, 0.2M MgCl2 0.1M NaCl, 0.05M Na cacodylate pH6.5