9QIH
Structure of response regulator LvrC from Leptospira
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-04 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.000040 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.289, 85.210, 111.271 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.010 - 2.600 |
| R-factor | 0.2058 |
| Rwork | 0.203 |
| R-free | 0.25250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.707 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.630 | 2.720 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.118 | 0.798 |
| Rmeas | 0.131 | 0.888 |
| Rpim | 0.056 | 0.379 |
| Number of reflections | 105097 | 2545 |
| <I/σ(I)> | 8.2 | 1.7 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 5 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.2 M sodium acetate trihydrate, 0.1 M sodium HEPES, 25 % w/v PEG 3350 |
