9QHR

Crystal structure of the second bromodomain of C. albicans Rsc1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-11
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9655
Spacegroup name	P 21 21 21
Unit cell lengths	66.183, 68.650, 130.269
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.500 - 1.500
Rwork	0.202
R-free	0.22610
Structure solution method	SAD
Data reduction software	XDS (February 5, 2021)
Data scaling software	XSCALE
Phasing software	SHELXD (2013/2)
Refinement software	PHENIX (1.15.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.500	1.610
High resolution limit [Å]	1.500	1.500
Rmeas	0.072	1.150
Number of reflections	181652	31375
<I/σ(I)>	14.1	1.2
Completeness [%]	99.0	94.7
Redundancy	6.03	3.6
CC(1/2)	0.999	0.628

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.2	277	0.1M potassium phosphate monobasic/sodium phosphate dibasic pH 6.2, 2-methyl-2,4-pentanediol 35% (v/v)