9QHL

ExsF in complex with BclA N-ter peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-11-13
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.98011
Spacegroup name	P 21 21 2
Unit cell lengths	125.353, 47.639, 97.905
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	62.680 - 1.930
R-factor	0.169
Rwork	0.167
R-free	0.20490
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.928
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	97.905	5.688
High resolution limit [Å]	1.928	1.928
Rpim	0.145
Number of reflections	38864	1943
<I/σ(I)>	5.8
Completeness [%]	93.1
Redundancy	10.3
CC(1/2)		0.458

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		294	0.1 M Sodium cacodylate 6.0 , 15 % w/v PEG 4000