9QGH
BV333 aminotransferase from Streptomyces sp. mutant F61C
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 113.15 |
| Detector technology | PIXEL |
| Collection date | 2024-03-12 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.852 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 99.747, 176.464, 111.259 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 88.230 - 1.310 |
| Rwork | 0.188 |
| R-free | 0.21020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.411 |
| Data reduction software | xia2 (3.9) |
| Data scaling software | XSCALE |
| Phasing software | MoRDa |
| Refinement software | REFMAC (5.8.0430 (refmacat 0.4.100)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 88.230 | 1.330 |
| High resolution limit [Å] | 1.310 | 1.310 |
| Rmeas | 0.069 | 2.047 |
| Number of reflections | 221445 | 7504 |
| <I/σ(I)> | 8.9 | |
| Completeness [%] | 94.8 | 64.8 |
| Redundancy | 6 | 2.9 |
| CC(1/2) | 1.000 | 0.300 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 292.15 | Morpheus Fusion Screen A7 |
