9QF7

PRMT2 in complex with aDMA analog

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	C 2 2 21
Unit cell lengths	65.727, 114.561, 132.721
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.560 - 2.270
R-factor	0.1941
Rwork	0.192
R-free	0.23370
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	0.874
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.21rc1_5015: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.560	2.340
High resolution limit [Å]	2.270	2.270
Rmerge	0.122	2.217
Rmeas	0.127	2.315
Rpim	0.035	0.654
Number of reflections	23265	1805
<I/σ(I)>	13.7	1
Completeness [%]	98.6	84.9
Redundancy	13.4	12.3
CC(1/2)	0.999	0.575

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.3	293	24% peg3350, 100 mM BT pH5.3, 0.1M Li2SO4