9QEO
Crystal structure of YTHDF2 in complex with compound 5 (AI-DF2-36)
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-04 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1 |
| Spacegroup name | P 65 |
| Unit cell lengths | 80.560, 80.560, 114.370 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.280 - 1.980 |
| R-factor | 0.2146 |
| Rwork | 0.212 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.831 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.100 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Number of reflections | 29208 | 4719 |
| <I/σ(I)> | 24.74 | |
| Completeness [%] | 99.2 | |
| Redundancy | 9.1 | |
| CC(1/2) | 0.999 | 0.973 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | 2.025 M Ammonium sulfate, 0.1 M Sodium citrate tribasic pH 4.9 |
