9QEL

Crystal structure of YTHDF2 in complex with compound 4 (AI-DF2-13)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-04
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 65
Unit cell lengths	80.470, 80.470, 113.890
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.100 - 1.860
R-factor	0.2189
Rwork	0.217
R-free	0.24830
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.780
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.970
High resolution limit [Å]	1.860	1.860
Number of reflections	34884	5666
<I/σ(I)>	16.56
Completeness [%]	98.9
Redundancy	6.9
CC(1/2)	0.997	0.746

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	1.975 M Ammonium sulfate, 0.1 M Sodium citrate tribasic pH 5.2