9QD0

Structure of Beilong paramyxovirus receptor binding protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-07
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9282
Spacegroup name	C 2 2 21
Unit cell lengths	87.980, 162.380, 226.070
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.230 - 3.500
R-factor	0.2474
Rwork	0.246
R-free	0.27320
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.565
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((1.21.1_5286: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	73.180	3.560
High resolution limit [Å]	3.500	3.500
Rmerge	0.140	0.950
Rmeas	0.150	1.000
Rpim	0.040	0.290
Number of reflections	20626	2888
<I/σ(I)>	13.8	2.5
Completeness [%]	99.8
Redundancy	12.9
CC(1/2)	1.000	0.870

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	Crystals grew in 0.2 M potassium thiocynate, 0.1 M sodium cacodylate pH 6.5, 8% w/v poly-gamma-glutamic acid polymer (PGA), 6% 1,5-diaminopentance di-HCl.