9QB5

AP2-associated protein kinase 1 (AAK1) bound to CKJB68

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00002
Spacegroup name	P 41 21 2
Unit cell lengths	74.386, 74.386, 260.510
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.990 - 2.000
R-factor	0.1994
Rwork	0.198
R-free	0.22670
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.828
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.000	1.860
High resolution limit [Å]	1.820	1.820
Number of reflections	66978	3859
<I/σ(I)>	12.7	1.4
Completeness [%]	100.0	99.3
Redundancy	12.9	10.2
CC(1/2)	0.997	0.626

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	293	0.2 M ammonium sulfate 30% PEG4000