9Q9V
Crystal structure of Borrelia burgdorferi BB0238-BB0323 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-12-09 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.918400 |
| Spacegroup name | P 65 |
| Unit cell lengths | 130.760, 130.760, 38.943 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.780 - 3.600 |
| R-factor | 0.20573 |
| Rwork | 0.199 |
| R-free | 0.33509 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.445 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.800 | 3.940 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rmerge | 0.104 | 0.541 |
| Number of reflections | 4620 | 1084 |
| <I/σ(I)> | 18.9 | 6.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 19.9 | |
| CC(1/2) | 0.999 | 0.979 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 294 | 0.01 M NiCl2 6H2O 0.1 M Tris pH 8.5 20% PEG 2000 MME |
