Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-05-11 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.99 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.104, 60.620, 41.931 |
| Unit cell angles | 90.00, 113.01, 90.00 |
Refinement procedure
| Resolution | 38.624 - 2.100 |
| Rwork | 0.232 |
| R-free | 0.29650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.445 |
| Data reduction software | xia2.multiplex |
| Data scaling software | xia2.multiplex |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.624 | 2.160 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 10082 | 809 |
| <I/σ(I)> | 4.8 | 1.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9 | |
| CC(1/2) | 0.959 | 0.300 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291.15 | 30% PEG 10000, 0.1 M TRIS HCl pH 8.5 |
