9Q9E

Crystal structure of a TELSAM-SUMO1 fusion protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-02-06
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.87313
Spacegroup name	P 21 21 21
Unit cell lengths	54.726, 99.692, 113.576
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.970 - 2.050
R-factor	0.2104
Rwork	0.209
R-free	0.23120
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.487
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1-4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.970	2.110
High resolution limit [Å]	2.050	2.050
Rpim	0.080	0.377
Number of reflections	39816	3081
<I/σ(I)>	7.2	2.2
Completeness [%]	100.0	100
Redundancy	6.7
CC(1/2)	0.994	0.404

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Na-phosphate/citric acid pH4.2, PEG600