9Q8O

Crystal structure of Hsp82-MD in complex with the CS domain of Sgt1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	193
Detector technology	PIXEL
Collection date	2021-05-14
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.0
Spacegroup name	I 2 2 2
Unit cell lengths	69.505, 117.308, 148.641
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	92.080 - 2.100
Rwork	0.199
R-free	0.24570
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	92.170	92.170	2.180
High resolution limit [Å]	2.100	4.520	2.100
Rmerge	0.031
Number of reflections	32066	3767	2390
<I/σ(I)>	17.8
Completeness [%]	89.5	99.7
Redundancy	2
CC(1/2)	0.999	1.000	0.578

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.04 M Magnesium chloride hexahydrate, 0.05 M Sodium cacodylate trihydrate, 5% v/v (+/-)-2-Methyl-2,4-pentanediol