9Q8D

CRYSTAL STRUCTURE OF HUMAN MONOGLYCERIDE LIPASE WITH LIGAND

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.99999
Spacegroup name	C 2 2 21
Unit cell lengths	90.544, 127.550, 62.947
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.270 - 1.540
R-factor	0.1968
Rwork	0.196
R-free	0.21850
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.851
Data reduction software	XDS
Data scaling software	SADABS
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419+SVN)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	73.830	1.640
High resolution limit [Å]	1.540	1.540
Rmerge	0.096	0.956
Number of reflections	54205	9191
<I/σ(I)>	7.14
Completeness [%]	100.0
Redundancy	7.39
CC(1/2)	0.999	0.641

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M MES pH 6.5, 15 % (v/v) isopropanol, 10 % (w/v) PEG MME 5000