9Q88

High-resolution structure of RNF38 RING domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-22
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97950
Spacegroup name	P 21 21 21
Unit cell lengths	31.820, 39.309, 53.208
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.620 - 1.200
R-factor	0.152
Rwork	0.151
R-free	0.16840
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.213
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.620	1.280
High resolution limit [Å]	1.200	1.200
Rmerge	0.053	0.270
Rpim	0.026	0.510
Number of reflections	30264	184
<I/σ(I)>	18.1	1.9
Completeness [%]	80.0
Redundancy	5.3
CC(1/2)	0.999	0.937

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	Molecular Dimensions; 0.09 M Halogens, 0.1 M Buffer System 1 pH 6.5, 37.5 % v/v Precipitant Mix 4